• Best coverage for known functional lncRNAs: Well-known LncRNAs characterized for biological functions or human disease association are comprehensively collected from the most updated public databases and landmark publications.

Figure 1. Some lncRNAs associated with cancers were collected from Bartonicek et al. 2016.

Figure 2. Some LncRNAs involved in cardiac pathways were collected from Devaux et al. 2015

• Comprehensive annotations: The LncRNAs that have been associated with diseases including cancers, cardiovascular diseases and neurodegenerative diseases are carefully categorized, annotated and cross-referenced.

Table1. The annotated information includes:
Class based on genomic location: [enhancer, antisense, intergenic, intronic, bidirectional…]
Target mRNA genes
Mechanisms: [signal, decoy, scaffold, guide, ceRNA, miRNA sponge, splicing …]
Biological functions: [cell cycle, differentiation, methylation, immune, metabolism…]
Diseases: [cancers, cardiovascular, neurodegenerative, kidney, diabetes, syndromes…]

    Annotation Example 1 – Cancer-associated lncRNA NBAT-1

Figure 3. Information of NBAT-1 annotated by Arraystar is shown in the green rectangular area.

    Annotation Example 2 – LncRNA Mhrt in cardiovascular diseases

 
Figure 4. Information of MHRT annotated by Arraystar is shown in the blue rectangular area.

    Annotation Example 3 – LncRNA BACE1-AS in neurodegenerative diseases

 
Figure 5. Information of BACE1-AS annotated by Arraystar is shown in the red rectangular area.

• A powerful tool for biomarker screening and validation: All the primer sets are optimized and fully validated experimentally using numerous sample types, to meet the needs of biomarker discovery.

Figure 6. A snippet of the lncRNAs on the PCR array with published biomarker potentials and utilities.

• Transcript-specific detection: Transcript-specific PCR primers are designed to target the specific exon or splice junction sites, for unambiguous and accurate detection of each individual LncRNA isoforms.

• Most reliable and accurate quantification: Each lncRNA is quantified at high accuracy and data quality, by using external controls (RNA Spike-In, and positive PCR control), Genomic DNA Control and six internal housekeeping genes. Normalization is performed by geometric means of multiple reference genes selected by experimentally validated algorithm, instead of a single reference gene.

• Fast and easy: Ready-to-use PCR array in a 384-well plate format. The entire process can be completed in less than 4 hours.